gps peptide substrate library plasmid pool (New England Biolabs)
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gps peptide substrate library plasmid pool
Gps Peptide Substrate Library Plasmid Pool, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 659 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gps+peptide+substrate+library+plasmid+pool/I-SceI/pm37735597-398-23-33
Average 97 stars, based on 659 article reviews
Gps Peptide Substrate Library Plasmid Pool, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 659 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gps+peptide+substrate+library+plasmid+pool/I-SceI/pm37735597-398-23-33
Average 97 stars, based on 659 article reviews
gps peptide substrate library plasmid pool - by Bioz Stars,
2026-09
97/100 stars
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Amplification:Article Title: Defining E3 ligase–substrate relationships through multiplex CRISPR screening Article Snippet: The DNA was concentrated by ethanol precipitation and then cloned into lentiCRISPR v2 (Addgene #52961) digested with BsmBI (NEB). .. The U6-sgRNA cassette was then amplified by PCR, purified by agarose gel electrophoresis (QIAEX II Gel Extraction Kit, Qiagen), and cloned into the Polymerase Chain Reaction:Article Title: Defining E3 ligase–substrate relationships through multiplex CRISPR screening Article Snippet: The DNA was concentrated by ethanol precipitation and then cloned into lentiCRISPR v2 (Addgene #52961) digested with BsmBI (NEB). .. The U6-sgRNA cassette was then amplified by PCR, purified by agarose gel electrophoresis (QIAEX II Gel Extraction Kit, Qiagen), and cloned into the Purification:Article Title: Defining E3 ligase–substrate relationships through multiplex CRISPR screening Article Snippet: The DNA was concentrated by ethanol precipitation and then cloned into lentiCRISPR v2 (Addgene #52961) digested with BsmBI (NEB). .. The U6-sgRNA cassette was then amplified by PCR, purified by agarose gel electrophoresis (QIAEX II Gel Extraction Kit, Qiagen), and cloned into the Agarose Gel Electrophoresis:Article Title: Defining E3 ligase–substrate relationships through multiplex CRISPR screening Article Snippet: The DNA was concentrated by ethanol precipitation and then cloned into lentiCRISPR v2 (Addgene #52961) digested with BsmBI (NEB). .. The U6-sgRNA cassette was then amplified by PCR, purified by agarose gel electrophoresis (QIAEX II Gel Extraction Kit, Qiagen), and cloned into the Gel Extraction:Article Title: Defining E3 ligase–substrate relationships through multiplex CRISPR screening Article Snippet: The DNA was concentrated by ethanol precipitation and then cloned into lentiCRISPR v2 (Addgene #52961) digested with BsmBI (NEB). .. The U6-sgRNA cassette was then amplified by PCR, purified by agarose gel electrophoresis (QIAEX II Gel Extraction Kit, Qiagen), and cloned into the Clone Assay:Article Title: Defining E3 ligase–substrate relationships through multiplex CRISPR screening Article Snippet: The DNA was concentrated by ethanol precipitation and then cloned into lentiCRISPR v2 (Addgene #52961) digested with BsmBI (NEB). .. The U6-sgRNA cassette was then amplified by PCR, purified by agarose gel electrophoresis (QIAEX II Gel Extraction Kit, Qiagen), and cloned into the Plasmid Preparation:Article Title: Defining E3 ligase–substrate relationships through multiplex CRISPR screening Article Snippet: The DNA was concentrated by ethanol precipitation and then cloned into lentiCRISPR v2 (Addgene #52961) digested with BsmBI (NEB). .. The U6-sgRNA cassette was then amplified by PCR, purified by agarose gel electrophoresis (QIAEX II Gel Extraction Kit, Qiagen), and cloned into the Cloning:Article Title: Defining E3 ligase–substrate relationships through multiplex CRISPR screening Article Snippet: The DNA was concentrated by ethanol precipitation and then cloned into lentiCRISPR v2 (Addgene #52961) digested with BsmBI (NEB). .. The U6-sgRNA cassette was then amplified by PCR, purified by agarose gel electrophoresis (QIAEX II Gel Extraction Kit, Qiagen), and cloned into the other:Article Title: Defining E3 ligase-substrate relationships through multiplex CRISPR screening. Article Snippet: Individual CRISPR/Cas9-mediated gene disruption experiments were performed using the lentiCRISPR v2 vector (Addgene #52961, deposited by Feng Zhang). |